Gradient pump instructions Shanghai Tongtian Biotechnology Co., Ltd. I. Working principle of gradient pump II. Gradient pump interface description III. Gradient pump interface setting IV. Example demonstration V. Gradient pump operation I. Gravity pump working principle Shanghai The same field of biological companies to use two constant current pump to achieve gradient control.One of the pumps A phase, another pump B phase, each flow 0.1ml / min increments or decrements. Operating principle shown in Figure 1.1: Figure 1.1 Gradient Pump Operation Schematic Diagram P1, P2, P3 and P4 are the set percentage P-T1, P-T2, P-T3 and P-T4 are the time t1 set from the previous percentage to the next percentage, t2, t3 and t4 are the delay time from the completion of one phase to the next phase. When the gradient is running, the total flow of the two pumps remains unchanged. For example, set the total flow to 50ml, pump A to increase, B If the flow of A pump at a time is 40.5ml, then the flow of B pump at this moment is 9.5ml. II. Interface Description of Gradient Pump 2.1 Initial Interface When you turn on the power, you will see Figure 2.1.1 The interface shown in Figure 2.1.1 at this time press the keyboard "0" key into the pressure of the cross-flow pump control system (see the specific operation of medium-voltage constant current pump instructions), press the keyboard "1" key to enter the gradient pump control system 2.2.2 Gradient pump control interface Figure 2.2.1 is the interface of the gradient pump control system 2.2.1 Gradient pump control interface Where: F00 - "F" means the total flow " 00 "set the flow rate (Grad10 is 0.1 ~ 10ml; Grad50 is 0.1 ~ 50ml; Grad200 is 1 ~ 200ml) â… 00: 000 -" â… "means the first stage;" 00 "means the percentage range set in this stage (0 ~ 999min) â…¡00: 000 - "â…¡" means the second phase; "00" means the percentage set in this phase , The range is from 0 to 100%; "000" means the time used to set up this phase of operation, the range is 0 ~ 999min â…¢00: 000 - "â…¢" means the third phase; "00" means the percentage set in this phase , The range is from 0 to 100%; "000" means the time used to set the operation in this phase range from 0 to 999 min â…£00: 000 - "â…£" means the third phase; "00" means the percentage set in this phase , The range is 0 ~ 100%; "000" means to set the running time in this phase, the range is 0 ~ 999min at this time if press the keyboard "P" key to enter the gradient pump delay interface. 2.2.2 delay pump gradient interface Figure 2.2.2 gradient pump delay interface where: DELAY1: 000 - "DELAY1:" said the first phase of the delay, "000" said the first phase of the delay When set the time, the range is 0 ~ 999minDELAY2: 000 - "DELAY2:" said the second phase of the delay, "000" said second phase delay set time, the range of 0 ~ 999minDELAY3: 000-- "DELAY3:" indicates the delay of the third stage, "000" indicates the delay of the third stage, and the range is 0 ~ 999minDELAY4: 000 - "DELAY1:" indicates the delay of the fourth stage, "000" It indicates the delay time of the fourth stage, the range is 0 ~ 999min. After setting the interface, it will automatically return to the gradient pump control interface. If no setting is needed, press the "RUN" key four times to automatically return to the gradient pump control interface. . Gradient pump interface settings Now we Grad50 series to illustrate the gradient pump interface settings.If the total flow of 50ml / min, A pump is decreasing, B pump is increasing.A pump from the first stage to go 100% To 80%, the time used is 10min, the delay is 10min; the second stage goes from 80% to 60%, the used time is 15min, the delay is 20min; the third stage goes from 60% to 50% The time is 20min, the delay is 30min; the fourth stage goes from 50% to 20%, the time used is 5min, and the delay time is 40min, then the control interface of A pump is set as follows: The setting of the control interface of Fig. 3.3 is as follows: Fig 3.4 The setting of the delay time interface is as follows: Fig 3.4 The curve of pump A running according to the above setting is shown in Fig.3.5: Fig.3.5B The curve of pump running is shown in Fig.3.6: Example Demo 4.1 Only run for a period 1) Stop after running, as shown in Figure 4.1.1: Figure 4.1.1A pump is: F50â… 80: 040â…¡00: 000â…¢00: 000â…£00: 000B pump is: F50â… 20: 040â…¡00: 000â…¢00: 000â…£00: 000 extension When the operation continues to run, for a period of time to stop. As shown in Figure 4.1.2: A pump is: F50â… 80: 040â…¡80: 100â…¢00: 000â…£00: 000B pump is: F50â… 20: 040 â…¡ 20: 100 â…¢00: 000 â…£00: 000 The delay time setting is DELAY1: 000DELAY2: 000DELAY3: 000DELAY4: 000 or set as: A pump is: F50â… 80: 040â…¡00: 000â…¢00: 000â…£00: 000B pump is: F50â… 20: 040â…¡00: 000â…¢00: 000â…£00 : 000 The delay screen is set to DELAY1: 100DELAY2: 000DELAY3: 000DELAY4: 0004.2 There are no delays in running the two phases 1), as shown in Figure 4.2.1 Figure 4.2.1A pump is: F50 â… 80: 040 â…¡ 30: 100 â…¢00: 000 â…£00: 000B pump is: F50 â… 20: 040 â…¡ 70: 100 â…¢00: 000 â…£00: 000 delay interface is set to DELAY1: 000DELAY2: 000DELAY3: 000DELAY4: 0002) Continue to run for some time, as shown in Figure 4.2.2: Figure 4.2.2A pump is: F50â… 80: 040â…¡80: 030â…¢30: 025â…£30: 150B pump is: F50â… 20: 040â…¡20: 030â…¢70: 025â…£70: 150 delay interface is set to DELAY1: 000DELAY2 : 000DELAY3: 000DELAY4: 000 Or set the delay interface, the specific settings are not listed .4.3 Running three stages 1) There is no delay in the middle, as shown in Figure 4.3.1: Figure 4.3.1A pump is: F50â… 80: 040â…¡60: 050â…¢020: 025â…£00: 000B The pump is: F50â… 20: 040â…¡40: 050â…¢80: 025â…£00: 000 delay interface is set as DELAY1: 000DELAY2: 000DELAY3: 000DELAY4: 0002) There is a delay in the middle, and continue to run for some time, as shown in Figure 4.3. 2 shown in Figure 4.3.2A pump is: F50â… 80: 040â…¡80: 020â…¢060: 050â…£20: 010B pump is: F50â… 20: 040â…¡20: 020â…¢40: 050â…£80: 010 delay interface is set to DELAY1: 000DELAY2: 000DELAY3: 000DELAY4: 1204.4. Run four stage